NO.

Item

A. Nucleic acid extraction

5-A1-1

A-1. Cell / Tissue nucleic acid extraction (DNA/RNA)

5-A1-2

A-2. Exosomal small RNA extraction

5-A1-3

A-3. FFPE DNA extraction

5-A1-4

A-4. FFPE RNA extraction

B. Quality Check

5-B1-1

B-1. nucleic acid QC

5-B1-2

B-2. Library QC

5-B1-3

B-3. FFPE DNA QC

Sequencing Platform

C. RNA Sequencing（included QC、Library preparation、Sequencing）

C-1. polyA RNA Sequencing (2*75 PE)

5-C1-1

C-1-1. 25 M reads

5-C1-2

C-1-2. 35 M reads

5-C1-3

C-1-3. 50 M reads

C-2. Ribominus RNA Sequencing (2*75 PE)

5-C2-1

C-2-1. 35 M reads

5-C2-2

C-2-2. 50 M reads

5-C2-3

C-2-3. 70 M reads

C-3. Ribominus RNA Sequencing (2*75 PE) For Bacteria RNA

5-C3-1

C-3-1. 10 M reads

5-C3-2

C-3-2. 20 M reads

C-4. RNA Exome Sequencing (2*75 PE) (Not included FFPE RNA extraction)

5-C4-1

C-4-1. 25 M reads

5-C4-2

C-4-2. 35 M reads

5-C4-3

C-4-3. 50 M reads

C-5. Small RNA Sequencing (1*75 SE)

5-C5-1

C-5-1. 10 M reads

5-C5-2

C-5-2. 15 M reads

5-C5-3

C-5-3. 20 M reads

C-6. Exosomal RNA Sequencing (1*75 SE)(Included exosomal RNA extraction)

5-C6-1

C-6-1. 10 M reads

5-C6-2

C-6-2. 15 M reads

5-C6-3

C-6-3. 20 M reads

D. DNA Sequencing（included QC、Library Preparation、Sequencing）

D-1. Whole Genome Sequencing (2*150 PE)

5-D1-1

D-1-1. 10 M reads, 3 G (For bacteria WGS)

5-D1-2

D-1-2. 15 M reads, 4.5 G

5-D1-3

D-1-3. 33 M reads, 10 G

5-D1-4

D-1-4. 50 M reads, 15 G

5-D1-5

D-1-5. 66 M reads, 20 G

5-D1-6

D-1-6. 83 M reads, 25 G

D-2. Whole Exome Sequencing-Agilent (2*150 PE)

5-D2-1

D-2-1. 100 x coverage

5-D2-2

D-2-2. 150 x coverage

5-D2-3

D-2-3. 200 x coverage

5-D2-4

D-2-4. 250 x coverage

D-3. Whole Exome Sequencing (2*150 PE)

5-D3-1

D-3-1. 100 x coverage

5-D3-2

D-3-2. 150 x coverage

5-D3-3

D-3-3. 200 x coverage

5-D3-4

D-3-4. 250 x coverage

D-4. FFPE-Whole Exome Sequencing (2*75 PE)

(included  FFPE  DNA extraction)

5-D4-1

D-4-1. 100 x coverage

5-D4-2

D-4-2. 150 x coverage

5-D4-3

D-4-3. 200 x coverage

5-D4-4

D-4-4. 250 x coverage

5-D4-5

D-4-5. 300 x coverage

E. Microbiome Sequencing（included QC、Library preparation、Sequencing）

5-E1-1

E-1. Microbiome-16S Library construction and      Sequencing

5-E1-2

E-2. TCR beta chain repertoire Library construction and Sequencing

5-E1-3

E-3. TCR alpha chain repertoire Library construction and Sequencing

5-E1-4

E-4. BCR heavy chain repertoire Library construction and Sequencing

5-E1-5

E-5. BCR light chain repertoire Library construction and Sequencing

F. Targeted sequencing: Precision Medicine Panel（included QC、Library preparation、Sequencing）

5-F1-1

F-1. Targeted DNA Panel-275 cancer-     related genes

5-Z-1

Customized Service

G. Single-cell sequencing（4 Sample / kit_included Library preparation and Sequencing

5-G-1

Single Cell Gene expression (5’)/40G per sample

5-G-2

Single Cell Gene expression (3’)/40G per sample

5-G-3

Single Cell Immune Profiling BCR/10G per sample

5-G-4

Single Cell Immune Profiling TCR/10G per sample

5-Z-2

Customized Study

H. Visium Spatial Gene Expression (included QC、Library preparation、Sequencing）

5-H-1

Visium CytAssist for FFPE Spatial Gene Expression 6.5mm kit and Library preparation

5-H-2

Visium CytAssist for FFPE Spatial Gene Expression 6.5mm Sequencing, 75G per sample

5-H-3

Visium CytAssist for FFPE Spatial Gene Expression 6.5mm Sequencing, 50G per sample

5-Z-3

Customized Service