基因體定序技術平台
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成果發表時，請於文章之致謝欄中載明。向本核心設施申請表列之各項服務或技術支援者，請簽署「同意書」，同意於成果發表時於文章的致謝欄 (Acknowledgment) 中載明。非核心設施表列之「服務項目」，以學術合作方式執行，其成果之分享等相關細節（如作者排序）則依一般學術合作慣例，由合作研究者自行擬定。原則於成果發表時，以本核心實驗室工作人員列名為共同作者。
基因體及定序技術平台收費標準（2021/09/08修正，2021/10/01生效）
(一)、定序技術平台：
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項目編號 |
服務項目 |
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A. 核酸萃取 |
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5-A1-1 |
A-1. 細胞/組織核酸萃取(DNA/RNA) |
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5-A1-2 |
A-2. Exosomal small RNA extraction |
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5-A1-3 |
A-3. FFPE DNA extraction |
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5-A1-4 |
A-4. FFPE RNA extraction |
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B. Quality Check |
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5-B1-1 |
B-1. 核酸QC |
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5-B1-2 |
B-2. Library QC |
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5-B1-3 |
B-3. FFPE DNA QC |
| Sequencing Platform | |
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C. RNA定序（含QC、建庫、定序） |
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C-1. polyA RNA Sequencing (2*75 PE) |
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5-C1-1 |
C-1-1. 25 M reads |
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5-C1-2 |
C-1-2. 35 M reads |
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5-C1-3 |
C-1-3. 50 M reads |
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C-2. Ribominus RNA Sequencing (2*75 PE) |
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5-C2-1 |
C-2-1. 35 M reads |
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5-C2-2 |
C-2-2. 50 M reads |
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5-C2-3 |
C-2-3. 70 M reads |
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C-3. Ribominus RNA Sequencing (2*75 PE) 適用Bacteria RNA |
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5-C3-1 |
C-3-1. 10 M reads |
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5-C3-2 |
C-3-2. 20 M reads |
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C-4. RNA Exome Sequencing (2*75 PE) (不含FFPE RNA extraction) |
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5-C4-1 |
C-4-1. 25 M reads |
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5-C4-2 |
C-4-2. 35 M reads |
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5-C4-3 |
C-4-3. 50 M reads |
|
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C-5. Small RNA Sequencing (1*75 SE) |
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5-C5-1 |
C-5-1. 10 M reads |
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5-C5-2 |
C-5-2. 15 M reads |
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5-C5-3 |
C-5-3. 20 M reads |
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C-6. Exosomal RNA Sequencing (1*75 SE)(含exosomal RNA 萃取) |
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5-C6-1 |
C-6-1. 10 M reads |
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5-C6-2 |
C-6-2. 15 M reads |
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5-C6-3 |
C-6-3. 20 M reads |
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D. DNA定序（含QC、建庫、定序） |
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D-1. Whole Genome Sequencing (2*150 PE) |
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5-D1-1 |
D-1-1. 10 M reads, 3 G (適用bacteria WGS) |
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5-D1-2 |
D-1-2. 15 M reads, 4.5 G |
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5-D1-3 |
D-1-3. 33 M reads, 10 G |
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5-D1-4 |
D-1-4. 50 M reads, 15 G |
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5-D1-5 |
D-1-5. 66 M reads, 20 G |
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5-D1-6 |
D-1-6. 83 M reads, 25 G |
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D-2. Whole Exome Sequencing-Agilent (2*150 PE) |
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5-D2-1 |
D-2-1. 100 x coverage |
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5-D2-2 |
D-2-2. 150 x coverage |
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5-D2-3 |
D-2-3. 200 x coverage |
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5-D2-4 |
D-2-4. 250 x coverage |
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D-3. Whole Exome Sequencing (2*150 PE) |
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5-D3-1 |
D-3-1. 100 x coverage |
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5-D3-2 |
D-3-2. 150 x coverage |
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5-D3-3 |
D-3-3. 200 x coverage |
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5-D3-4 |
D-3-4. 250 x coverage |
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D-4. FFPE-Whole Exome Sequencing (2*75 PE) (含 FFPE DNA extraction) |
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5-D4-1 |
D-4-1. 100 x coverage |
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5-D4-2 |
D-4-2. 150 x coverage |
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5-D4-3 |
D-4-3. 200 x coverage |
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5-D4-4 |
D-4-4. 250 x coverage |
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5-D4-5 |
D-4-5. 300 x coverage |
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E. Microbiome定序（含QC、建庫、定序） |
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5-E1-1 |
E-1. Microbiome-16S Library construction and Sequencing |
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5-E1-2 |
E-2. TCR beta chain repertoire Library construction and Sequencing |
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5-E1-3 |
E-3. TCR alpha chain repertoire Library construction and Sequencing |
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5-E1-4 |
E-4. BCR heavy chain repertoire Library construction and Sequencing |
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5-E1-5 |
E-5. BCR light chain repertoire Library construction and Sequencing |
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F. Targeted sequencing: 精準醫學Panel（含QC、建庫、定序） |
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5-F1-1 |
F-1. Targeted DNA Panel-275 cancer- related genes |
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5-Z-1 |
客製化檢驗 |
| G. 單細胞定序 Single-cell sequencing（4樣本為一套組建庫、包含基本定序） | |
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5-G-1 |
Single Cell Gene expression (5’端)/40G數據量 |
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5-G-2 |
Single Cell Gene expression (3’端)/40G數據量 |
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5-G-3 |
Single Cell Immune Profiling_BCR/10G數據量 |
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5-G-4 |
Single Cell Immune Profiling_TCR/10G數據量 |
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5-Z-2 |
客製化檢驗 |
| H. 空間轉錄體分析_Visium Spatial Gene Expression (含QC、建庫、定序) | |
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5-H-1 |
Visium CytAssist for FFPE Spatial Gene Expression 6.5mm (第二代空間轉錄體分析-石蠟包埋樣本)試劑及建庫套組 |
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5-H-2 |
Visium CytAssist for FFPE Spatial Gene Expression 6.5mm定序數據 75G |
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5-H-3 |
Visium CytAssist for FFPE Spatial Gene Expression 6.5mm定序數據 50G |
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5-Z-3 |
客製化檢驗 |
註：此服務收費標準為未稅價，欲開發票者須外加5%稅金
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